关键词: 肺癌, 抑癌基因p53, 蛋白基因产物（PGP9.5）, 转录因子（SOX2）, 肿瘤相关基因编码蛋白（GAGE7）, 解旋酶（GBU4-5）, 黑色素瘤抗原（MAGE A1）

Abstract:

Background and purpose: The pathogenesis of lung cancer is diverse, however once confirmed, the best time for treatment is often missed. In this paper, the correlation between tumor suppressor gene (p53), protein gene product (PGP9.5), transcription factor (SOX2), tumor-associated gene protein (GAGE7), helicase (GBU4-5) and melanoma antigen (MAGE A1) protein levels in lung cancer tissues and peripheral blood of patients was discussed, and its application value in the diagnosis and treatment of lung cancer was analyzed. Methods: A total of 100 lung cancer patients in Fudan University Shanghai Cancer Center from May 2018 to May 2019 were enrolled, including 25 cases of clinical TNM stage Ⅰ, 45 cases of stage Ⅱ, 30 cases of stage Ⅲa, 80 cases of non-small cell lung cancer and 20 cases of small cell lung cancer. Following surgical removal of intact tumor tissues and cancer-adjacent tissues, immunohistochemical staining was used to detect positive rates of above-mentioned 6 proteins, real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect their gene expression levels, and enzyme-linked immunosorbent assay (ELISA) was performed to detect antibody positive rates in peripheral serum. Results: The positive rates and gene expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were significantly higher compared with adjacent tissues (P<0.05). Correlation analysis between p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 proteins in tumor tissues and serum showed that the positive rates and gene expression levels of P53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 were correlated with TNM stage and differentiation level (P<0.05). There was no significant difference in gender, age, tumor diameter and pathological type (P>0.05). The protein expression levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 in tumor tissues were consistent with their expression levels in peripheral blood (P>0.05). Conclusion: Positive expressions of p53, PGP9.5, SOX2, GAGE7, GBU4-5 and MAGE A1 protein in lung cancer tissues and peripheral serum were closely related to TNM staging and differentiation.

Key words: Lung cancer, Tumor suppressor gene (p53), Protein gene product (PGP9.5), Transcription factor (SOX2), Tumor-associated gene protein (GAGE7), Helicase (GBU4-5), Melanoma antigen (MAGE A1)